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Sponsored by: Honors Program
Seminars: CANCELLED -- TO BE RESCHEDULED IN 2017-2018 Following Single mRNAs from Birth to Death in Living Cells
Start Date/Time: 6/12/2017 4:00 PM
End Date/Time: 6/12/2017 5:00 PM
Location: Alumni Hall B
Description: CANCELLED -- TO BE RESCHEDULED IN 2017-2018 Imaging has been an essential tool to analyze the dynamic properties of RNA. Newly developed new technologies in optical microscopy and novel methods for RNA tagging have allowed us to detect and track individual mRNA molecules in single living cells, yielding insights that could not have been obtained through any ensemble measurement. These approaches employ a plethora of imaging techniques, ranging from multiphoton microscopy, high-speed real-time widefield microscopy, single molecule tracking, super-registration microscopy and fluorescence fluctuation analysis. The kinetics of the key elements of RNA metabolism: initiation, elongation, termination, export, cytoplasmic trafficking, localization, translation and decay are now tractable at the single molecule level in real time in living cells. For instance it was possible to measure the initiation frequency and elongation rates of single mRNA transcription or translation, or the probability and time to be transported through a nuclear pore. We have been dedicated most recently to developing and implementing these technologies to further the understanding of dynamics of mRNA regulation in living tissues, particularly neural tissues of a transgenic mouse where the mRNAs synthesized from targeted genes contain stem-loops from a phage bound by a fluorescent capsid. By observing RNA in its native environment, we may find new regulatory mechanisms. Supported by funding from NIH.
Contact: Josephine Markiewicz
Phone: 263-3815
Email: Josephine.Markiewicz@nyumc.org
Speaker: Robert H. Singer, PhD
Speaker Institution: Albert Einstein College of Medicine
Speaker Institution Location: Bronx, NY
Speaker Position: Professsor and Co-Chair, Anatomy & Structural Biology
Addtl. Info: Co-Director, Gruss-Lipper Biophotonics Center
Seminar Host: Ruth Lehmann
Refreshments: No
Fee Required: No
Fee Amount:

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